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. 2010 Dec 8;5(12):e14249. doi: 10.1371/journal.pone.0014249

Figure 2. Western blot analysis of proteins encoded by amplified genes.

Figure 2

A. Over-expression of PHKG1. The gene was amplified in tumour 4 and not amplified in tumours 7 and 21. B. Over-expression of EGFR. Controls (C): extracts from 2 glioblastomas without EGFR amplification expressing the wild-type form of the protein. Tumours 4 and 7: full-length wild-type EGFR. Tumour 21: EGFRvIII variant. Tumour 22: EGFRvIII variant plus full-length wild-type (the apparent small size of the wild-type form, also present as a minor product in tumour 7, could correspond to a protein with posttranslational modifications). Receptor degradation products are visible in the tumour 7 lane. C. Over-expression of EGFR in tumour 26. The two truncated proteins expressed in this tumour are of the EGFRvI variant form. Actin: loading control.