Table 1. Relationships between EGFR gene amplification and mRNA and protein accumulation.

Characteristic	Case 4	Case 7	Case 21	Case 22	Case 26	Case 30
Histologya	ODA	GBM	GBM	ODA	GBM	GBM
DNA amplificationb	9	8	32	55	250c	30
mRNA over-expression d	8	13	24	38	300	27
Protein over-expressione	10	120	40	50+50f	150+100f	NAg
pEGFR form	wt	wt	vIII	wt+vIII	vI	NAg

ODA: oligodendroglioma; GMB: glioblastoma multiforme.

^aHistological type.

^bmean level of amplification.

^camplicon 3, unexpressed amplicons 1 and 2 were both amplified 8-fold

^dMean level of mRNA expression.

^eMean value of protein accumulation.

^fThe 2 bands were quantified independently (see Figure 2).

^gprotein extract not available.