Figure 3. Timing and connectivity of retino-thalamic and thalamocortical visual pathways are established and not altered 12 h after ocular drug administration.

(a) Representative images of antero-posterior coronal sections of the retina (top), optic chiasm (middle) and dLGN (bottom) after intraocular injection of Cholera Toxin conjugated with 594- red fluorophore and Epibatidine 1 mM (n = 3). The dye transport established connectivity between the retina and dLGN at E16. (b) Neurofilament (NF) staining showed an unaltered timing and connectivity of the thalamo-cortical pathway in E16 treated brains (n = 3 for each group). Abbreviations: dLGN dorsal lateral geniculate nucleus, dTh dorsal thalamus and NCx neocortex.