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. 2010 Dec 8;5(12):e15227. doi: 10.1371/journal.pone.0015227

Table 3. Oligonucleotides used in this study.

Name Sequence (5-3′)
RI-Xb-SD-espF CCGGAATTCTCTAGAAAGAGGCATAAATTATGCTTAATGGAATTAGTA
SfiI-espF CTGCACCTGAGCCATGGCCGGCTGGGCCGCTGCGATACCTACAAGCTGCCGCCCTA
SfiIVamy CTTGTAGGTATCGCAGCGGCCCAGCCGGCCATGGCTCAGGTGCAGCTG
RI-stop-E CCGGAATTCTCATTAGGCCGGTTCCAGCGGATCCGGATACGGCAC
Vhh-SfiI2 GTCCTCGCAACTGCGGCCCAGCCGGCCATGGCTCAGGTGCAGCTGGTGGA
Vhh-NotI2 GGACTAGTGCGGCCGCTGAGGAGACGGTGACCTGGGT
NdeI-espF CCGGATCCATATGCTTAATGGAATTAGTAACGCTGCTTCT
EcoRI-espF GGTGCGAATTCGCTGCGATACCTACAAGCTGCCGCCCTA
EcoRI-TEM GCGGCAGCTTGTAGGTATCGCAGCGAATTCGCACCCAGAAACGCTGGTGA
BamHI-tetra ATGCGTCCGGCGTAGAGGATCCACAGGACGGGT
NdeI-espFVamy GGGAATTCCATATGCTTAATGGAATTAGTAACGCTGCT
EcoRIVamy-espF CCGGAATTCGCGGCCGGTTCCAGCGGATCCGGATA