. 2010 Dec 8;5(12):e14266. doi: 10.1371/journal.pone.0014266

Table 1. Completeness test of two 454 sequence assemblies using BLASTN ^a.

Assembly	Unique sequences	Significant hits (E ≤10⁻⁵)	Identity of ≥25 overlapping bases100% identical >95% identical
Newbler
Singleton	157,070	493,800	2,109	8,818
Contig	31,114	32,713	579	2,419
Total	188,184	526,513	2,688	11,237
SeqMan
Singleton	129,486	44,111	917	2,414
Contig	63,602	44,979	339	1,489
Total	193,088	89,090	1,256	3,903

Each unique 454 singleton or contig was aligned against all other unique sequences within the same assembly.

Table 1. Completeness test of two 454 sequence assemblies using BLASTN a.