Table 2. Correctness test of two assemblies using BLASTN a.
| Assembly | Unique sequences | Significant hits (E ≤10−5)EF EA+LS | Full length identity b≥90% (EF) ≥80% (EA+LS) | ||
| Newbler | |||||
| Singleton | 157,070 | 3,207 | 5,306 | 400 | 344 |
| Contig | 31,114 | 1,713 | 3,613 | 534 | 548 |
| Total | 188,184 | 4,920 | 8,919 | 934 | 892 |
| SeqMan | |||||
| Singleton | 129,486 | 1,766 | 3,005 | 129 | 207 |
| Contig | 63,602 | 2,813 | 5,677 | 593 | 1,250 |
| Total | 193,088 | 4,579 | 8,682 | 722 | 1,457 |
a
Each unique 454 singleton or contig was aligned against all available Sanger sequences of Eisenia fetida (EF) (2231 SSH +104 GenBank dbEST), Eisenia andrei (EA) and Lumbricus spp. (LS) (1108 EA +17225 LS). If one unique 454 sequence hit more than one Sanger sequences, only the most significant one was counted.
b
The full length (≥100 bases) is that of the subject or the query, whichever is shorter.