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. 2010 Dec 8;5(12):e15318. doi: 10.1371/journal.pone.0015318

Figure 3. Immuno EM localisation of Rab27a on the viral envelope of isolated HCMV viral particles.

Figure 3

Isolated viral particles from BJ1 YFP-Rab27a cells HCMV infected for 5 days were permeabilised with saponin and labelled with antibodies against YFP (A) and HCMV tegument viral protein pp28 (B), and 10 nm protein-A gold. (C) General morphology of HCMV virions by negative staining with 2% uranyl acetate. When viral particles were partially disrupted, uranyl acetate revealed the nucleocapsids. Scale bar, 50 nm.