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. 2010 Nov 18;21(12):1735–1739. doi: 10.1089/hum.2010.077

FIG. 1.

FIG. 1.

Overexpression of SERCA1a in the mdx diaphragm protects from eccentric contraction–induced loss of force production. (a) Immunoblotting of diaphragm homogenates demonstrated 69% less SERCA1a in the mdx diaphragm (red column) in comparison with C57 Bl/6 controls (navy column). AAV-mediated gene transfer increased SERCA1a content in mdx/SERCA1a diaphragm (green column) to 52% of control. (b) The muscle fiber size distribution was unaffected by SERCA1a overexpression. (c) An increase in the proportion of type IIA fibers and a decrease in the proportion of type IIX fibers were observed in the mdx/SERCA1a group. (d) The percentage of centrally nucleated fibers (CNFs) was reduced from 25% in the mdx diaphragm to 13% in the mdx/SERCA1a diaphragm. (e) Force production was measured following a series of eccentric contractions (ECC). Beginning at the second contraction, there was a significant attenuation of the force loss in mdx/SERCA1a diaphragm in comparison with the untreated mdx diaphragm. Data represent means ± SD. *P < 0.05, mdx vs. C57/Bl6. #P < 0.05, mdx vs. mdx/SERCA1a. Color images available online at www.liebertonline.com/hum.