Figure 6. Effect of bisphosphonates on calcification of rat aortas in culture.
Top: Aortas were incubated in DMEM containing 3.8 mM phosphate, 3.75 U/ml of alkaline phosphatase, 25 μM warfarin, and the indicated concentrations of bisphosphonate for 9 days. Results are the means±s.e. of seven aortic rings. Bottom: Injured and uninjured aortas were incubated in DMEM with 3.8 mM phosphate, with or without 3.75 U/ml of alkaline phosphatase, and the indicated concentrations of pamidronate for 9 days. Results are the means±s.e. of at least three aortic rings.