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. 2010 Nov 23;11(1):161. doi: 10.1186/1465-9921-11-161

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Copyright ©2010 Oswald-Richter et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sarcoidosis BAL T cells proliferate in response to multiple mycobacterial antigens. Sarcoidosis BAL cells were CFSE-labeled and activated with neoantigen (KLH), ESAT-6, katG, Ag85A, sodA, and HSP. Day 4 post-activation, antigen-specific proliferation of CD4+ and CD8+ T cells was assessed by gating on CD3+CD4+ T cells or CD3+CD8+ T cells and analyzing the CFSE expression of each subset by flow cytometry. Percent proliferation is indicated by bracket above peaks. Similar results were found in the three other tested subjects.