Table 1.
Primers for the amplification of the full-length genome sequence of PPV4.
| # | Primers | Sequences | Position* | Tm (°C) |
|---|---|---|---|---|
| 1 | PF356 | 5' TGACGCAGTACAGACCGACGAGA 3' | 356 | 60 |
| PR1197 | 5' AATGCAAGTGCAAGCCACCTTTT 3' | 1197 | ||
| 2 | PF1050 | 5' AGTAATCTGGTAATCGCTGTTCG 3' | 1050 | |
| PR1942 | 5' ATGTTAGTCTTTCCTGTTGTGGC 3' | 1942 | ||
| 3 | PF1568 | 5' GCTGGTGGATAACAACATCTGCT 3' | 1568 | |
| PR2553 | 5' GTTTCTTCTTTCTCGGTGCTTCT 3' | 2553 | ||
| 4 | PF2530 | 5' AAGAAGCACCGAGAAAGAAGAAA 3' | 2530 | |
| PR3413 | 5' AAATCTAAGGGACAAGGCAAACG 3' | 3413 | ||
| 5 | PF3363 | 5' AGATACTAAGAAAGACAAGGTGGAG 3' | 3363 | |
| PR4263 | 5' AATAATAGAAGGTATAGCGTCTCCA 3' | 4263 | ||
| 6 | PF3918 | 5' ACCTGCTCCTCCATCTTCTCCAC 3' | 3918 | |
| PR4897 | 5' GGCCGTCATCATACATTCTGCTC 3' | 4897 | ||
| 7 | PF4219 | 5' ACTTACTGTTCTATGATGTCTGGAG 3' | 4219 | |
| PR5862 | 5' ATATCATCTGCGGTGTCTGGG 3' | 5862 |