Figure 5.

Expression of SLC7A6 and SLC7A7 in monocytes, alveolar macrophages (AM) and fibroblasts obtained from the LPI subject. After RNA extraction and reverse transcription, cDNA was employed as template for qPCR with SLC7A6/y+LAT2 and SLC7A7/y+LAT1 primers. mRNA level, normalized for RPL15 gene, is expressed as number of molecules (see Methods). For normal cells, data are means ± SEM of 9 (monocytes) or 3 (AM and fibroblasts) independent experiments. For the LPI patient, bars are means ± SD of 6 (monocytes and fibroblasts) or 4 (AM) determinations.