Figure 6.

Effect of GM-CSF on the differentiation of LPI monocytes to macrophages. Normal and LPI monocytes were differentiated to Monocyte-Derived Marcophages (MDM) by 5 d incubation in RPMI supplemented with rGM-CSF (10 ng/ml). Panels A and B. Phase contrast images of normal (A) and LPI (B) MDM. × 100. Panel C. Induction of macrophage differentiation markers in normal and LPI MDM. In freshly isolated (control) or rGM-CSF-treated (MDM) monocytes RNA was extracted and cDNA employed as template for qPCR with primers for the indicated genes. The values are normalized to that of RPL (see Eq 1 in Methods). For normal cells, data are means ± SEM of 5 independent experiments (n = 5 normal donors). For the LPI patient, data are means ± SD of 4 determinations.