Figure 6.
Effects of ACSL5 gene knockdown on mitochondrial metabolism and tumorigenesis. A, change in cellular O2 consumption; and B, change in intracellular ATP level in HCT116 and LOVO cells transduced with ACSL5 shRNA relative to control scramble shRNA (n = 5). Bars, stdev. p < 0.05 by Student's t test comparing cells transduced with ACSL5 shRNA versus control scramble shRNA. C, cellular and mitochondrial ROS levels in HCT116 cells transduced with ACSL5 shRNA versus control scramble shRNA, as measured by the fluorescent dyes CM-H2DCFDA and MitoSOX.