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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Neurogastroenterol Motil. 2010 Oct 1;23(1):76–e8. doi: 10.1111/j.1365-2982.2010.01603.x

Figure 4.

Figure 4

Histograms of real-time two-step RT-PCR analysis of inflammatory mediator mRNA expressions 3 hours postoperatively (SM: intestinal manipulation). The mRNA of the three inflammatory indicators were quantified (IL-6, TNF-α and ICAM-1) in unoperated vehicle controls, glycine controls and after surgical manipulation with and without glycine pretreatment of the surgically manipulated animals. Each inflammatory mRNA was markedly induced by surgical manipulation and this induction was significantly blunted by glycine pretreatment (n = 6-8 per group, mean ± SEM; F-test and Bonferroni post-hoc group comparisons with * = p-values < 0.05).