Table 1.
Nucleotide sequences of oligonucleotide primers. Slopes and correlation coefficients of the primer standard curves.
| Target gene |
Primer sequences (5′to 3′) | MgCl2 | Slope | Correlation- coefficient |
|---|---|---|---|---|
| GAPDH | Sense: ATGGCACAGTCAAGGCTGAGA | 3 | −3.197 | 0.996 |
| Antisense: CGCTCCTGGAAGATGGTGAT | ||||
| COX-2 | Sense: CTCTGCGATGCTCTTCCGAG | 5 | −3.494 | 0.991 |
| Antisense: AAGGATTTGCTGCATGGCTG | ||||
| ICAM-1 | Sense: CGTGGCGTCCATTTACACCT | 3 | −3.404 | 0.991 |
| Antisense: TTAGGGCCTCCTCCTGAGC | ||||
| IL-6 | Sense: GCCCTTCAGGAACAGCTATGA | 5 | 3.360 | 0.997 |
| Antisense: TGTCAACAACATCAGTCCCAAGA | ||||
| iNOS | Sense: GGAGAGATTTTTCACGACACCC | 3 | −3.518 | 0.999 |
| Antisense: CCATGCATAATTTGGACTTGCA | ||||
| TNF-α | Sense: GGTGATCGGTCCCAACAAGGA | 5 | −3.021 | 0.997 |
| Antisense: CACGCTGGCTCAGCCACTC |