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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Lab Invest. 2010 Aug 16;91(2):216–231. doi: 10.1038/labinvest.2010.146

Figure 4. Characterization of immune cell infiltration in the limb muscle, diaphragm and the heart.

Figure 4

Figure 4

a, Representative photomicrographs of immunohistostaining in the dog spleen. For CD8+ T cell and the secondary antibody alone, high power images of the boxed areas are shown. Positive cells stain in dark purple color. b, Representative photomicrographs of immunohistostaining for B cells, CD4+ T cells and CD8+ T cells in the limb muscle of dystrophic corgi dogs. Yellow arrowhead, a rare B cell. c, photomicrographs of immunohistostaining for neutrophils and macrophages in the limb muscle and diaphragm of dystrophic corgi dogs. Top panel, low magnification images; Bottom panel, high magnification images. Purple arrowhead, neutrophil in dystrophic limb muscle; Green arrowhead, neutrophil in dystrophic diaphragm muscle. Neutrophils are less abundant than macrophages. d, Representative photomicrographs of immunohistostaining for neutrophils and macrophages in the limb muscle of normal and carrier dogs. Arrow, centrally located nuclei; Blue arrowhead, a neutrophil in the carrier limb muscle; Red arrowhead, a macrophage in the carrier limb muscle. e, photomicrographs of immunohistostaining for neutrophils and macrophages in the heart of normal and dystrophic corgi dogs. Top panel, low magnification images; Bottom panel, high magnification images. Left two columns, normal heart; Right two columns, dystrophic heart. Pink arrowhead, sporadic neutrophil in the normal heart; Orange arrowhead, sporadic macrophage in the normal heart; Scale bar applies to all the images in the same row.