Table 1.

Breeding record

Parent Information	Male Normal	Femalea Normal	Male Normal	Femaleb Carrier
Mating	2		6
Litter size	4, 7		2c, 3, 5, 6d, 6, 9
Average litter size	5.5		5.2
Puppy number
Total	11		31
Male	5 (45.5%)		14 (45.2%)
Female	6 (54.5%)		16 (51.6%)
Unknown gender	0		1 (3.2%)e
Puppy genotype
Normal	11 (100%)		18 (58.1%)
Affectedf	0 (0%)		5 (16.1%)
Carrier	0 (0%)		7 (22.6%)
Unknown	0 (0%)		1 (3.2%)e
Neonatal deathg
Normal	0		3h
Affected	NA		2 (stillborn)
Carrier	NA		1 (stillborn)
Unknown genotype	NA		1 (stillborn)e

^aTwo females (Adrian and Madonna) were used in breeding.

^bFour female (Crash, Rachel, Monica, and Bitty) were used in breeding. Three litters were from carrier Monica.

^cThis litter was born on 7/6/2007. Both puppies were female (one is normal, the other is a carrier) and both were stillborn.

^dThis litter was born on 6/25/2007. Only two survived from this litter (1 normal male and 1 carrier). Two affected males were stillborn. One normal male died at day 3 and one normal female died at day 8.

^eThis was a stillbirth and puppy’s gender was not recorded and tissue is not available for genotyping.

^fAll affected are male.

^gNeonatal death also include five cases of stillbirth. Four deaths were from the first litter of carrier Rachel (see c) and two from the first litter of carrier Crash (see d). These deaths occurred in 2007. These two carriers were not used for breeding since then. One stillbirth occurred in later 2009 and it was the third litter from carrier Monica. This was also the largest litter (total 9 puppies).

^hOne normal male is stillborn, one normal male died at day 3 and one normal female died at day 8.