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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: J Thromb Haemost. 2010 Dec;8(12):2778–2788. doi: 10.1111/j.1538-7836.2010.04094.x

Figure 5. Reducing activity of ADAMTS-13 truncation mutants.

Figure 5

VWF multimers captured with thiol beads were incubated with various concentrations of full-length rADAMTS-13, M, MDTCS, TCSTCu, or STCu domain for 1 hr at 37°C. VWF released from the beads was separated by 6% SDS-PAGE and detected by immunoblotting using an anti-VWF antibody. A full-length rADAMTS-13 protein (50 nM) was used as a positive control and IAA-treated rADAMTS-13 as a negative control for WT rADAMTS-13. The figure represents 3-5 separate experiments.