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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Biochemistry. 2010 Nov 17;49(49):10354–10356. doi: 10.1021/bi101711a

FIGURE 1.

FIGURE 1

The C≡N stretching bands of W3C-CN obtained in the presence (open circles) and absence (open triangles) of CaM (50 mM HEPES buffer, pH 7.4, 30 mM CaCl2). For the former case, the concentrations of W3C-CN and CaM were estimated to be 1–2 mM. Lines are respective fits of these data to a Lorentzian function with the following parameters: for W3C-CN ν = 2236.5 cm−1 and Δν = 10.7 cm−1, for W3C-CN/CaM ν = 2231.2 cm−1 and Δν = 11.7 cm−1.