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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Clin Cancer Res. 2010 Oct 4;16(23):5722–5733. doi: 10.1158/1078-0432.CCR-10-1693

Fig. 3.

Fig. 3

(A) Representative light microscopy images showing immunohistochemical staining of F4/80 to identify infiltrating macrophages/microglia in a high-grade tumor at (i) low (100X) and (ii) high (400X) magnification, and a low-grade tumor at (iii) low (100X) and (iv) high (400X) magnification, and (v) rat IgG isotype control (100X). (B) In the PDGF-B alone, and PDGF-B + Bcl-2 mice, macrophage infiltration was significantly higher in high-grade tumors than in low-grade tumors (** P < 0.05). (C) In PDGF-B + Bcl-2 mice with high-grade gliomas that survived past 90 days had significantly lower levels of macrophage infiltration than those that succumbed to intracranial tumors before 90 days (** P = 0.003). (D) Mean degree of macrophage infiltration in individual mice in the PDGF-B + Bcl2 group bearing high-grade tumors plotted against overall survival time, showing long-term survival of all animals with macrophage infiltration of < 20%. (* = animals sacrificed after surviving past 90 days. Error bars represent standard error of the mean.)