Fig. 5.
(A) Kaplan-Meier survival curve showing improved survival in PDGF-B and Bcl-2 mice treated with the p-STAT3 inhibitor WP1066 compared with untreated controls. (B) WP1066 treatment significantly reduced levels of p-STAT3 expression. Representative light microscopy images show immunohistochemistry of p-STAT3 (brown nuclear staining) in a high-grade glioma from a vehicle control mouse and a WP1066-treated mouse (400X). (C) WP1066 also suppressed F4/80-positive macrophage infiltration. Representative light microscopy images showing immunohistochemistry of F4/80 (brown cytoplasmic staining) demonstrating infiltrating macrophages/microglia in a high-grade glioma from a vehicle control mouse and a WP1066-treated mouse (400X). (D) WP1066 did not suppress the number of intratumoral FoxP3-positive cells. Representative light microscopy images showing immunohistochemistry of FoxP3 (brown staining) from a vehicle control mouse and a WP1066-treated mouse (400X). (* P < 0.05).