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Semiquantitative RT-PCR analysis of selected genes in primary cultured astrocytes following 24 h of exposure to aluminum (Al-gly; 0.1 mM) and Tu (5 μg/ml). Tu, but not Al-gly, up-regulated the expression of calreticuline, calnexin, and stanniocalcin 2. Total RNA was analyzed by multiplex RT-PCR for the mRNA expressions of calreticuline, calnexin, stanniocalcin 2, and GAPDH. The results are representative of three independent analyses from two replicate cultures
