Fig. 7.

a Semiquantitative RT-PCR analysis of the immunoglobulin-binding protein/glucose-regulated protein 78 (BiP/Grp78) gene in primary cultured astrocytes following exposure to aluminum (Al-gly; Al) and Tu for the indicated time periods. Total RNA was analyzed by RT-PCR for the mRNA expression of Bip/Grp78. Al-gly did not up-regulate the expression of Bip/Grp78. Cells were treated with Tu (5 μg/ml) for 48 h or with Al-gly for 48 or for 12 h followed by 7 days of culture in normal medium. The results are representative of three independent analyses from two replicate cultures. b Relative intensities of the bands