Table 1.
Target gene | Forward primer | Reverse primer | Product size (bp) |
---|---|---|---|
Ire1α | CCCAAATGTGATCCGCTACT | TTGAGAGAATGCAGGTGTGC | 183 |
Ire1β | ACTGTGGATCCAGGAAGTGG | TGGCTGTGTCTTGGTAGCTG | 193 |
ATF6α | GGCCAGACTGTTTTGCTCTC | CCCATACTTCTGGTGGCACT | 215 |
ATF6β | GGGTCCATCCCCTGATAGTT | CACCTTGGATGAGGACCACT | 185 |
PERK/Eif2ak3 | CGGATTCATTGAAAGCACCT | ACGCGATGGGAGTACAAAAC | 194 |
Bbc3/PUMA | GCCCAGCAGCACTTAGAGTC | TGTCGATGCTGCTCTTCTTG | 191 |
Bip/Grp78 | TGGAGTTCCCCAGATTGAAG | GCGCTCTTTGAGCTTTTTGT | 199 |
CHOP/GADD153 | CCTAGCTTGGCTGACAGAGG | CTGCTCCTTCTCCTTCATGC | 196 |
Stanniocalcin 2 | TCCAGCAATTAGGGAAATGG | CCCAGCTCTGTTCACACTGA | 230 |
Calnexin | GGCTAGACGACGAACCTGAG | AGGCTTCCATTTGCCCTTAT | 188 |
Calreticulin | AGGCTCCTTGGAGGATGATT | TCCCACTCTCCATCCATCTC | 207 |
OASIS | ACCTGGACCACTTTGTGGAG | TGGTGTCCTCCATCTTGACA | 210 |
See text and abbreviation list for the full names of the target genes