Table 1.
Cytotoxic effects of atisine-type C20-diterpenoid alkaloids against A549 cell lines
| Compound | 1 μg/mla | 5 μg/mla | IC50 (μM) |
|---|---|---|---|
| Control | 1.00 | 1.00 | – |
| 1 | 1.07 ± 0.16 | 1.00 ± 0.05 | ND |
| 2 | 1.12 ± 0.16 | 0.93 ± 0.04 | ND |
| 3 | 1.01 ± 0.03 | ND | ND |
| 4 | 0.98 ± 0.02 | ND | ND |
| 5 | 1.02 ± 0.01 | ND | ND |
| 6 | 0.88 ± 0.06* | ND | ND |
| 7 | 0.96 ± 0.06 | ND | ND |
| 8 | 0.98 ± 0.06 | 0.86 ± 0.03 | ND |
| 9 | 0.65 ± 0.06* | ND | 4.07 ± 0.00 |
| 10 | 0.94 ± 0.01 | ND | ND |
| 11 | 1.02 ± 0.01 | 1.05 ± 0.04 | ND |
| 12 | 0.65 ± 0.08* | ND | 2.20 ± 0.11 |
| 13 | 0.89 ± 0.03 | 0.56 ± 0.04* | ND |
| 14 | 0.66 ± 0.04* | 0.15 ± 0.04* | 3.68 ± 0.30 |
| 15 | 1.04 ± 0.14 | 0.25 ± 0.06* | ND |
| 16 | 0.54 ± 0.14* | 0.03 ± 0.01* | 1.72 ± 0.03 |
| 17 | 0.93 ± 0.04 | 0.83 ± 0.03 | ND |
| 18 | 0.75 ± 0.08* | ND | 5.08 ± 0.15 |
| 19 | 0.93 ± 0.05 | 0.47 ± 0.04* | ND |
| 20 | 0.86 ± 0.11 | 0.11 ± 0.08* | ND |
| 21 | 0.98 ± 0.11 | 0.42 ± 0.10* | ND |
| 22 | 0.74 ± 0.14* | 0.03 ± 0.01* | 2.66 ± 0.21 |
| 23 | 1.05 ± 0.06 | 0.91 ± 0.05 | ND |
| 24 | 0.95 ± 0.04 | 0.90 ± 0.02 | ND |
| 25 | 0.59 ± 0.10* | ND | 4.24 ± 0.00 |
| 26 | 1.05 ± 0.06 | ND | ND |
| 27 | 0.69 ± 0.09* | ND | 4.67 ± 0.08 |
| 28 | 1.03 ± 0.05 | 1.17 ± 0.31 | ND |
| 29 | 1.09 ± 0.19 | 0.72 ± 0.07* | ND |
| 30 | 1.06 ± 0.19 | 0.64 ± 0.11* | ND |
| 31 | 0.98 ± 0.14 | 0.35 ± 0.04* | 11.42 ± 0.71 |
| 32 | 1.04 ± 0.25 | 0.50 ± 0.06* | ND |
| 33 | 1.09 ± 0.06 | 1.04 ± 0.05 | ND |
| 34 | 0.93 ± 0.19 | 0.44 ± 0.11* | ND |
| 35 | 0.72 ± 0.11* | 0.21 ± 0.04* | 5.44 ± 0.41 |
| 36 | 0.64 ± 0.06* | ND | 3.75 ± 0.14 |
| 37 | 0.91 ± 0.19 | 0.37 ± 0.10* | ND |
| 38 | 0.92 ± 0.23 | 0.89 ± 0.04* | ND |
| 39 | 0.75 ± 0.22 | 0.27 ± 0.24* | 3.02 ± 0.47 |
| Pemetrexed | 0.14 ± 0.05* | 0.13 ± 0.04* | 0.48 ± 0.07 |
ND not determined
aEach value is the mean ± SD of three determinations
* p < 0.05, indicating significant difference from the control value