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. 2003 Dec 1;100(25):15247–15252. doi: 10.1073/pnas.2434676100

Fig. 1.

Fig. 1.

Purification of 26RFa from frog brain. (A) After prepurification on Sep-Pak cartridges, the brain extract was analyzed on a semipreparative reverse-phase HPLC column. The fractions denoted by the bars contained NPFF-LI, and fraction 61 was selected for further purification. (B) Final purification step of 26RFa on an analytical Vydac C18 reverse-phase HPLC column. The column was eluted with a linear gradient of increasing acetonitrile concentration (denoted by the dashed line), and individual peaks were collected by hand. The arrow shows the peak containing NPFF-LI. The arrowheads show where peak collection began and ended.