Fig. 4.
Phl p 5-specific IgG1 and IgG2a antibody subclass responses after vaccinations. (A) Sera of mice from day 22 after three intradermal immunizations with all three constructs (pCMV-F1/Tet, pCMV-F1, or pCMV-P5) rendered similar tires of IgG1 and IgG2a antibodies. (B) Mice receiving four times particle bombardment by gene gun showed higher IgG1 levels in all three DNA immunized groups (B). **P < 0.01.