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. Author manuscript; available in PMC: 2010 Dec 9.
Published in final edited form as: Immunol Lett. 2008 Dec 25;122(1):68–75. doi: 10.1016/j.imlet.2008.12.002

Fig. 5.

Fig. 5

Functional IgE antibodies measured by RBL cell mediator release assay. (A) rPhl p 5 protein, but none of the mimotope-DNA constructs pCMV-F1 and pCMV-F1/Tet induced significant Phl p 5-specific IgE synthesis after i.d. immunization. (B) In contrast, gene gun injection triggered IgE induced β-hexosaminidase release in all experimental groups. Results are presented as relative percentage of total β-hexosaminidase released after addition of 10% Triton X-100. Samples showing more than 3-fold deviation from the end of the box were defined as extremes and marked with numbers and circles. Numbers represent sample numbers in the order given by the statistical program used. *P < 0.05, **P < 0.01.