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. 2006 Sep;2(3):151–154. doi: 10.4103/0972-9941.27729

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© Journal of Minimal Access Surgery

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cross polarization microscopical (CPM) and immunohistochemical features of human fascial tissue according to Junqueira.[12] CPM of Sirius red-stained section of normal fascia with a collagen type I/III ratio of 14 - (A); and specimen of recurrent incisional hernia fascia with a collagen type I/III ratio of 3.6 - (B). For the detection of MMP-2, we used rabbit polyclonal, 1:1000, from Biomol (Hamburg, Germany) as primary antibody; and goat anti-rabbit, 1:500, from Dako (Glostrup, Denmark) as secondary antibody. Positive cytoplasmatic expression of MMP-2 in granuloma adjacent to mesh filaments - (C, D) (positive stained cells marked with black arrows). (Magnification 400× in images IIIA - IIID).