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. 2010 Dec;131(4):513–524. doi: 10.1111/j.1365-2567.2010.03324.x

Figure 3.

Figure 3

Effects on bone marrow-derived dendritic cells (BMDC) production are differentially dependent on MyD88 and Tir-domain-containing adaptor-inducing interferon (TRIF). (a) MyD88+/+ and MyD88−/− bone marrow cells (5 × 105 cells) were cultured for 6 days with granulocyte–macrophage colony-stimulating factor (GM-CSF) in the presence of lipopolysaccharide (LPS), Jap, X31 or PR8. ***P < 0·0001, determined using the Student's t-test. (b) TRIF+/+ and TRIF−/− bone marrow cells (5 × 105) were cultured for 6 days with GM-CSF in the presence or absence of Poly I, Poly I:C, LPS, cytosine–phosphate–guanosine (CpG) oligodeoxynucleotide (ODN), Jap, X31 or PR8. Surface markers were analysed by flow cytometry. The results are based on data for 10 000 gated events. The data shown are representative of two similar experiments. *P < 0·05, **P < 0·001 and ***P < 0·0001, determined using the Student's t-test.