Figure 2.

YVL-specific CD8 T cells display an effector memory phenotype like other cytomegalovirus (CMV) -specific CD8 T cells. Donor Y26 peripheral blood mononuclear cells were stimulated for 6 hr with synthetic peptides and surface stained for cell surface markers followed by cytoplasmic staining with anti-interferon-γ monoclonal antibody (IFN-γ mAb). Perforin expression was also determined by cytoplasmic staining. IFN-γ-positive CD8 T cells were gated and analysed for membrane phenotype. Values in the upper right quadrant indicate the frequency of IFN-γ-producing cells that express the given marker. Data are shown for three different responses: YVL (tetr^– response) VTE (tetr⁺ response) and NLV (tetr⁺ response).