Figure 6.

CD8 T-cell clones specific for YVL peptide were tested for the ability to recognize altered peptide ligands. These included YVL peptide analogues with changed human leucocyte antigen (HLA) -A2-binding anchor residues at position 2 (V→L, designated YLL) and/or position 9 (M→V, designated V9). Assays were performed either in the absence (a) or presence (b) of CD8 blocking monoclonal antibodies. Representative data from two clones are shown. Effector : target ratios used were 2 : 1 in these assays. (c) Second, YVL-specific clones were screened against different concentrations of YVL peptide variants with single alanine substitutions at positions 1–9 (A1–A9). The unaltered wild-type (WT) YVL peptide is indicated as grey bars. The data shown are derived from YVL clone 4 with similar results observed using other YVL clones. Mean values from triplicate wells are shown (the standard deviation was < 5% of maximal lysis).