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. 2010 Dec;131(4):570–582. doi: 10.1111/j.1365-2567.2010.03329.x

Figure 2.

Figure 2

Induction of interleukin-12 (IL-12) and activation markers after co-culture of naive bone-marrow-derived dendritic cells (BMDC) with IL-12 p40−/− BMDC. (a) Fluorescence staining of intracellular IL-12 after direct stimulation of BMDC with CpG oligodeoxynucleotides (ODN) or after co-culture of naive BMDC with CpG ODN-stimulated IL-12 p40−/− BMDC. Upper panels: IL-12 production by unstimulated wild-type (wt) BMDC and after direct stimulation with CpG ODN. Lower panels: CFSE-labelled naive IL-12 p40−/− BMDC and co-culture of wild-type BMDC with CFSE-labelled IL-12 p40−/− BMDC loaded with Leishmania major lysate. Representative dot blots of three independent experiments. (b) Production of IL-12 after direct stimulation of BMDC and after co-culture of naive BMDC with stimulated IL-12 p40−/− BMDC. Native, unstimulated BMDC; Lysate, stimulation with L. major lysate; L+C, stimulation with L. major lysate and CpG ODN; CpG, stimulation with CpG ODN; n.d., no cytokine secretion detected. Representative enzyme-linked immunosorbent assay of three independent experiments. (c) Expression of major histocompatibility complex (MHC) II on unlabelled naive BMDC after co-culture with CFSE-labelled BMDC stimulated with L. major lysate and CpG ODN. The CFSE-intermediate population demonstrates the uptake of particles from CFSE-labelled stimulated BMDC by naive BMDC. L+C, stimulation with L. major lysate and CpG ODN. Representative dot blots of three independent experiments.