Fig. 4.

Single-cell RT-PCR of Arabidopsis thaliana plasma membrane intrinsic protein 1;1 (AtPIP1;1)-transformed and control pollen grain protoplasts from lily (Lilium longiflorum). Fluorescent as well as control pollen grain protoplasts were collected and immediately frozen in liquid nitrogen. cDNA was generated by reverse transcription with AtPIP1;1-specific reverse primer, followed by PCR with an AtPIP1;1-specific primer pair. Amplified product was separated by agarose gel electrophoresis, blotted onto a membrane and detected using a digoxigenin-labelled specific AtPIP1;1 probe. Lane 1, water control; lanes 2–5, individual, transformed protoplasts; lanes 6–8, nontransformed protoplasts.