. Author manuscript; available in PMC: 2010 Dec 9.

Published in final edited form as: J Membr Biol. 2007 Jun 14;215(2-3):111–123. doi: 10.1007/s00232-007-9011-6

Table 2.

Values of the osmotic parameters Pos and β obtained for different protoplast types. The water permeability coefficient was calculated by three methods: (a) fitting the transient osmotic volume with Eq. 4, (b) using the “initial rate” method and (c) using uncorrected values for the protoplast volume neglecting the non-osmotic volume and fitting the raw volume data with Eq. 4

Protoplast source	Pos (μm s⁻¹)			β
	Fit with Eq. 4	“Initial rate” method	Non-osmotic volume ignored	fit with Eq. 4	Boyle-van’t Hoff plot (upper limit of 95% confidence)
Lilium longiflorum pollen grain	6.59 ± 2.26	4.87 ± 1.68^*	5.31 ± 2.22	0.27 ± 0.12	0.420 (0.520)
Lilium longiflorum pollen tube	15.74 ± 5.05	12.12 ± 6.00	6.59 ± 3.17^*	0.29 ± 0.08	0.047 (0.403)
Nicotiana tabacum mesophyll	5.75 ± 2.89	3.94 ± 1.13^*	4.23 ± 2.52^*	0.27 ± 0.13	0.017 (0.210)
Arabidopsis thaliana mesophyll	11.31 ± 6.04	8.15 ± 4.63^*	6.45 ± 3.23^*	0.32 ± 0.11	0.004 (0.144)

Significantly different results (p < 0.05) obtained with methods (b) and (c) compared to method (a). The relative non-osmotic volume β was calculated from individual volume transients using Eq. 10 or by interpolation from Boyle-van’t Hoff plots with the upper limit of the 95% confidence in parentheses (Fig. 2)