Skip to main content
. Author manuscript; available in PMC: 2011 Nov 10.
Published in final edited form as: J Am Chem Soc. 2010 Nov 10;132(44):15820–15830. doi: 10.1021/ja107716r

Figure 3.

Figure 3

Effect of the N43A and N43S mutations on the stability of PYP. Thermal unfolding (A) and denaturant titration (B) of wtPYP (solid line), N43S PYP (dashed line), and N43A PYP (dotted line) probed using visible absorbance at 446 nm for wtPYP and 441 nm for the mutants. The data for the thermal unfolding of N43A PYP are truncated at the point where aggregation started to occur. The lines in (B) are fits of the denaturant titration data as single transitions. (C) Comparison of the denaturant titration of N43A PYP as detected by absorbance at 441 nm (circles) and aromatic fluorescence emission at 350 nm (squares).