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. 2010 Nov 24;6(7):700–715. doi: 10.7150/ijbs.6.700

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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(A) Involvement of caspases in endogenous and BMP4 meditated cell death of WERI-RB-1 cells. Treatment of WERI-RB1 cells with Boc-D-fmk, a broad spectrum caspase inhibitor, revealed that endogenous apoptosis is caspase independent, while BMP4 mediated apoptosis in these retinoblastoma cells is at least partially caspase-dependent. Values are means of three independent experiments (n=3) ± s.e.m. *** P<0.001 calculated by one way Annova test and Newman-Keuls Post test. (B) Western Blot analyses of changes in Bax protein levels following 24hr BMP4 treatment of WERI-Rb1 cells. Levels of the pro-apoptotic protein Bax remain unchanged (B).