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. 2010 Nov 25;6(7):719–729. doi: 10.7150/ijbs.6.719

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Fig 5 — GEP KD mice display a reduction in enamel mineralization. A) Von Kossa staining showed a reduction of mineralization in the GEP knockdown mice (KD, right panel) compared to the age matched wild type (WT, left panel). B) MTT assay data showed an increase in cell proliferation in the ameloblast cell line with all 3 concentrations of recombinant GEP protein, although there was a significant difference only at the concentration of 200 ng. C) Recombinant GEP protein increased levels of cell markers for ameloblast cell differentiation: ameloblastin, amelogenin, and enamelin. These values were normalized by GAPDH. (Data are mean ± SEM, n=4, p <0.05).