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. 2010 Dec 2;6(7):756–768. doi: 10.7150/ijbs.6.756

Figure 2.

Figure 2

Schema of the NHEJ reporter assay. A: The pEGFP-N1 vector backbone was PCR amplified using reverse primer P1 and forward primer P2. B: pCMV-pax6in-eGFP plasmid in which the pax6 fragment and SV40poly(A) was ligated with the pEGFP-N1 vector backbone, in order to prevent the CMV promoter from driving eGFP expression. C: The insertion fragment was removed from the pCMV-pax6in-eGFP plasmid through double digestion, and the linearized plasmid was injected into zebrafish embryos or eggs. D: The linearized vector was rejoined by NHEJ in zebrafish embryos or eggs.