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. 2010 Dec 2;6(7):756–768. doi: 10.7150/ijbs.6.756

Figure 3.

Figure 3

Zebrafish embryos were microinjected with circular type plasmid pCMV-pax6in-eGFP and linearized plasmid which had the insertion fragment removed from between the CMV promoter and the eGFP reporter gene. A: Zebrafish embryos microinjected with circular type plasmid pCMV-pax6in-eGFP (no fluorescence was observed). B: Zebrafish embryos microinjected with linearized plasmid which had the insertion fragment removed from between the CMV promoter and the eGFP reporter gene in the pCMV-pax6in-eGFP plasmid. Re-expression of eGFP was observed as the embryo emitted fluorescence.