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. 2010 Dec 2;6(7):756–768. doi: 10.7150/ijbs.6.756

Figure 4.

Figure 4

Gel electrophoresis of the PCR products amplified from the genomes of six fluorescent zebrafish embryos which were microinjected with linearized pCMV-pax6in-eGFP plasmids. A band of 531 bp length was amplified from all the fluorescent zebrafish embryo genomes, which represented the expected product from a religation event between the CMV promoter and eGFP. Lanes 1-6: PCR products of the amplified beta-actin gene, used for genome quality control. Lanes 1'-6': PCR products of the junction sites between the CMV promoter and eGFP. -: negative control in which the template was the wild type zebrafish genome DNA. +: positive control in which the template was circular type pCMV-pax6in-eGFP plasmid. M: DNA molecular weight marker.