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. 2010 Dec 2;6(7):756–768. doi: 10.7150/ijbs.6.756

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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NHEJ effects in unfertilized zebrafish eggs. 1, 4, 7 junction site PCR product amplified from the genomes of fluorescent embryos microinjected with linearized DNA. 2, 5, 8 junction site PCR product amplified from homogenates of unfertilized eggs incubated with linearized DNA. 3, 6, 9 linearized vector used as the PCR template. 1-3: XhoI+HindIII linearized DNA; 4-6: KpnI +SacI linearized DNA; 7-9: HindIII+EcoRI linearized DNA. M: DNA molecular weight marker. DNA, 10: template was the wild type zebrafish genome. +: template was the pCMV-pax6in-eGFP plasmid. A band of 531 bp length was amplified from all the fluorescent zebrafish embryo genomes, which was the expected product for the event of religation between the CMV promoter and eGFP.