Skip to main content
. 2010 Dec 14;1(5):e00285-10. doi: 10.1128/mBio.00285-10

TABLE 1 .

Soluble sugar production following digestion of hatched wheat straw (17-h incubation period) by various enzyme combinationsa

Enzyme combination Amt of sugar producedb
Arabinose Xylose Xylobiose Cellobiose Xylotriose
Wild-type free enzymes 6.8 ± 0.1 (45.7) 6.8 ± 0.3 (45.7) 19 ± 0.6 (68.6) 46.7 ± 1.4 (137.1) 4.5 ± 0.4 (10.9)
Divalent cellulase- and xylanase-containing designer cellulosomes 6.5 ± 0.5 (42.8) 5.3 ± 0.3 (34.2) 12.7 ± 1.5 (45.7) 58.7 ± 1.9 (168.6) 11.3 ± 0.7 (27.1)
Tetravalent cellulase/xylanase-containing designer cellulosome 6.8 ± 0.2 (45.7) 7.8 ± 0.1 (51.4) 31.6 ± 0.7 (111.4) 61.5 ± 1 (180) 12.7 ± 0.6 (30.6)
a

 Data were obtained by HPLC analysis. The absence of glucose was confirmed by using a glucose assay kit. Values for xylose were corroborated using a xylose assay kit. An unidentified peak, present only after enzymatic treatments, eluted at ~3.9 min (between the xylose and xylobiose peaks) and was possibly a modified monosaccharide.

b

 Values are shown in milligrams per gram of substrate (mean ± standard deviation). The values in parentheses are micromoles per gram of substrate.