TABLE 1.
Physical properties and LCAT kinetic data for reconstituted lipoprotein particles
| Reconstituted Lipoproteina | ApoA-I per Particlea | POPC:FC:ApoA-I Ratio (n = 6)a | appKmb | Vmaxb |
|---|---|---|---|---|
| μM | nmol CE/h/LCAT unit | |||
| WT rHDL | 2 | 87:10:1 | 1.17 ± 0.23 | 1.87 ± 0.08 |
| ±3 ± 0.4 | ||||
| Mixed rHDL | 2 | 87:10:1 | 1.79 ± 0.12c | 1.75 ± 0.09 |
| ±6 ± 1.0 | ||||
| S36A rHDL | 2 | 84:10:1 | 6.88 ± 0.23c | 2.19 ± 0.41 |
| ±4 ± 0.5 |
The reconstituted lipoprotein particles are WT apoA-I, S36A apoA-I, and 1:1 molar mixture of the two proteins. apoA-I, apolipoprotein A-I; LCAT, CE, cholesteryl ester; lecithin:cholesterol acyltransferase; WT, wild-type.
a
All reconstituted lipoprotein particles were prepared and characterized according to McManus et al. (33).
b
appKm and Vmax values were determined from Lineweaver-Burk plots.
c
Differences that are statistically significant (P < 0.05) were calculated by Student's t-test.