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. 2011 Jan;52(1):113–124. doi: 10.1194/jlr.M010264

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Copyright © 2011 by the American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 4. — LC/ESI-MS/MS analysis of 15-d-PGJ₂ (A) and a mixture of 15-d-PGJ₂-like compounds (deoxy-J₂-IsoPs) obtained from the nonenzymatic oxidation of AA in vitro (B). A: LC/ESI-MS/MS analysis of a chemically synthesized 15-d-PGJ₂ employing SIM (upper chromatogram) and CID (lower product ion spectrum). B: LC/ESI-MS/MS analysis of 15-d-PGJ₂-like compounds obtained from the in vitro oxidation of AA employing SIM for the precursor ion m/z 315 (upper chromatogram) and CID (lower product ion spectrum) indicative of putative 15-d-PGJ₂.