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. 2011 Jan;52(1):113–124. doi: 10.1194/jlr.M010264

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Copyright © 2011 by the American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 9. — Analysis of putative deoxy-J₂-IsoPs in vivo by LC/MS/MS using SRM. LC/MS/MS analysis of (A) deoxy-J₂-IsoPs regioisomers and (B) the 15-series deoxy-J₂-IsoPs (rac-15-d-PGJ₂) generated in vivo esterified in liver phospholipids in rats after treatment with CCl₄ and (C) chemically synthesized [²H₄]15-d-PGJ₂ (d₄-15-d-PGJ₂). The mass spectrometer was operated in the negative ion mode with SRM for the precursor-to-product transitions of m/z 315–271 for all deoxy-J₂-IsoPs regioisomers (chromatogram A), m/z 315–203 for rac-15-d-PGJ₂ (chromatogram B), and m/z 319–275 for d₄-15-d-PGJ₂ (chromatogram C).