Skip to main content
. 2011 Jan;52(1):98–103. doi: 10.1194/jlr.M009480

Fig. 2.

Fig. 2.

CK2 inhibitor TBB attenuates effects of OxPAPC on induction of NRF2 and GCLM mRNA. A: The CK2 inhibitor TBB prevents induction of NRF2 by OxPAPC. At 30 min before stimulation with OxPAPC (130 µmol/l) HUVECs were pretreated with vehicle or TBB (20 µmol/l). After 2 h of stimulation, cell lysates were prepared and probed with NRF2 antibodies using Western blotting. B: NRF2/ARE DNA binding assay. Binding of NRF2 from control and OxPAPC-stimulated HUVEC nuclear extracts to immobilized oligonucleotide representing the consensus NRF2 binding site was quantified with an ELISA-based TransAM NRF2 kit and is expressed as an optical density of 450 nm. C, D, E: Effect of TBB on induction of GCLM mRNA levels by OxPAPC in different types of ECs (HAECs, HCAECs, and HUVECs, respectively). Before stimulation with OxPAPC (130 µmol/l, 6 h) cells were pretreated with TBB (20 µmol/l) for 30 min. Stimulation was stopped by addition of Trizol after 6 h. GCLM mRNA expression was quantified by real-time qPCR and normalized to β2-microglobulin mRNA levels.