Figure 2.

Pamidronate and Zoledronate treatment sensitizes tumor cells to lysis by Vγ9Vδ2 T cells. MCF-7 breast carcinoma (A), PC-3 prostate carcinoma (B) and SaOS-2 osteosarcoma (C) cells were treated with Pamidronate (black rectangles, 100 µM) and Zoledronate (black-filled circles, 100 µM) or left untreated (open circles) for 16-18 hours. ⁵¹Cr-labeled tumor cells were co-cultured with Vγ9Vδ2 T cells for 4 hours at E:T ratios ranging from 30:1 to 1.8:1. Error bars indicate % cytotoxicity±SE. Statistical significance: @, P < 0.005; *, P < 0.0001; #, P < 0.002. Shown are the means of three independent experiments performed using γδ T cells from 3 different donors against each target cell line.