Figure 4.

Time-lapse imaging of Zoledronate-treated MCF-7 cells co-cultured with Vγ9Vδ2 T cells. MCF-7 cells were pretreated with Zoledronate (100 µM) for 16-18 hours and co-cultured with Vγ9Vδ2 T cells at a 1:2 ratio for 4 hours at 37˚C. Snapshots from continuous time-lapse imaging taken during the last 1 hour are shown. The images were taken at 30 seconds interval (a-h). The yellow arrow indicates a single MCF-7 cell being targeted by Vγ9Vδ2 T cells (black arrow). Changes in tumor cell morphology are observed (e-h) finally leading to lysis of the tumor cell by Vγ9Vδ2 T cells (g-h). Representative images from one of four independent experiments are shown.